Markers dupw167 and AFSr13 were validated on 21 durum wheat genotypes. The map order dupw167– Sr13– AFSr13– gwm427 was deduced from the recombination data. Marker gwm427 mapped proximal to AFSr13 in two populations, and these markers were monomorphic on one population each. Marker dupw167, previously reported to be linked with Sr13, mapped 2.3–5.7 cM distal to Sr13 in four F 3 populations. The STS marker AFSr13, derived from an AFLP fragment, mapped at 3.4–6.0 cM proximal to Sr13 across three mapping populations. ![]() ![]() We used the AFLP approach to identify markers linked closely with Sr13. To combine Sr13 with other genes that produce a similar phenotype, linked markers would be required. It remains a candidate for developing new cultivars with diverse combinations of stem rust resistance genes. tritici pathotype Ug99 (TTKSK) and its derivatives. Stem rust resistance gene Sr13, found frequently in tetraploid wheats, was tested effective against Puccinia graminis f.
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